Difference Between EIA and ELISA Immunoassay Techniques

What is the main difference between EIA and ELISA immunoassay techniques?

Why would you choose to use ELISA instead of EIA?

Main Difference Between EIA and ELISA

One reason to use an ELISA is its high specificity and sensitivity, allowing for accurate and precise measurements.

EIA and ELISA are both immunoassay techniques used for detecting and quantifying substances in biological samples. While EIA stands for Enzyme Immunoassay, ELISA stands for Enzyme-Linked Immunosorbent Assay.

The main difference between EIA and ELISA lies in the way they are performed. EIA involves binding an enzyme-labeled antibody to the substance of interest, which produces a detectable signal. On the other hand, ELISA involves binding an antigen or antibody of interest to a solid surface, and then detecting the bound substance using an enzyme-labeled secondary antibody.

One reason to choose ELISA over EIA is its high specificity and sensitivity. ELISAs are commonly used in research, clinical diagnostics, and pharmaceutical industries to detect and quantify substances such as hormones, proteins, antibodies, and drugs with precision and accuracy.