Why is it important to spray all samples with peroxide before spraying them with the indicator solutions?

The Significance of Using Peroxide in Sample Preparation

Peroxide is a crucial component in the sample preparation process before staining with indicator solutions. The main reason why all samples need to be sprayed with peroxide first is to bleach the samples, making them colorless before they are stained by the indicators. Peroxide belongs to a class of chemicals where two oxygen atoms are linked by a single covalent bond, which makes it an effective bleaching agent.

When peroxide is applied to the samples, it works by reducing the color bodies in the pulp. It does this by oxidizing the carbonyl group, which ultimately results in the samples becoming colorless. Once the samples are bleached, the indicator solution is then applied. This process allows the indicator to penetrate the samples more effectively, highlighting important tissue features and enhancing tissue contrast.

This entire process of using peroxide before indicator staining is essential in obtaining accurate and clear results during sample analysis. It ensures that the tissue characteristics are properly showcased and easily distinguishable under the microscope.

Why is peroxide sprayed on all samples before applying indicator solutions?

Peroxide is sprayed on all samples before applying indicator solutions to bleach the samples, making them colorless and enabling the indicators to highlight tissue features effectively.