DNA Polymerase III: The Superhero of DNA Replication

What is DNA Polymerase III responsible for in DNA replication?

Choose the correct option:
A. Adds nucleotides to the growing DNA chain
B. Removes RNA primer
C. Seals gaps on the lagging strand

Answer:

The correct options are A, B, and C.

DNA Polymerase III is a multi-protein complex that plays a vital role in DNA replication. It is like the superhero of the DNA replication process, carrying out multiple essential tasks to ensure accurate and efficient replication of the genetic material.

First and foremost, DNA Polymerase III adds nucleotides to the growing DNA chain in the 5'->3' direction. This activity is crucial for extending the DNA strand during replication and synthesizing new DNA molecules.

Secondly, DNA Polymerase III is responsible for removing the RNA primer. RNA primers provide a starting point for DNA replication, but they need to be replaced with DNA bases. This enzyme takes care of removing the primer and replacing it with newly synthesized DNA.

Lastly, DNA Polymerase III seals the gaps on the lagging strand. During DNA replication, the lagging strand is synthesized in short fragments called Okazaki fragments. This enzyme is in charge of sealing the gaps between these fragments to create a continuous DNA strand.

In conclusion, DNA Polymerase III exhibits 5'->3' polymerase activity, 3'->5' exonuclease activity, and acts as a proofreader during DNA replication. Its multitasking abilities make it an indispensable player in the intricate dance of DNA replication.